Journal: Communications Biology

Article Title: Platelet extracellular vesicles preserve lymphatic endothelial cell integrity and enhance lymphatic vessel function

doi: 10.1038/s42003-024-06675-8

Figure Lengend Snippet: CellVue + human PEVs (1 × 10 7 ) or vehicle control were injected in the dermis of the footpad in wild-type mice. Lymph nodes (LNs) draining the site of injection (green boxes) and LNs located at a distal site (beige boxes) were collected 15 min and 2 h post-injection. Then, they were ( a ) visualized with a fluorescence imaging scanner (IVIS Lumina II) or ( b ) digested with collagenase D to determine the percentage of cells containing EVs. c The concentration of free EVs within the LNs were assessed by flow cytometry using anti-mouse MHCII and anti-human CD62P antibodies. d Two hours post-PEV injection, popliteal lymphatic vessels draining the injection site were also collected, harvested and stained with anti-human CD41a antibody (red) and DAPI (blue) to be imaged with a confocal microscope (white scale bar, 20 µm). The lymphatic contraction capacity was measured in vivo 48 h after the injection of ( e ) PEVs or ( f ) rbEVs in Ldlr -/- , and ( g ) after injection of PEVs in wild-type mice. h The lymph nodes (N-D, beige; D, green) and ( i ) the aorta were also collected at 48 h and visualized with fluorescent imaging. Each point represents a mouse ± SEM. N-D: non-draining; D: draining; LN: lymph nodes; EVs: extracellular vesicles. PEVs: platelet extracellular vesicles. rbEVs: red blood cell extracellular vesicles. Created with Biorender.com .

Article Snippet: Furthermore, in a final set of mice, non-draining LNs, draining LNs and the aorta were harvested and imaged individually in a fluorescence imaging scanner (IVIS Lumina II, Caliper Life Sciences).

Techniques: Control, Injection, Fluorescence, Imaging, Concentration Assay, Flow Cytometry, Staining, Microscopy, In Vivo